福建省蛋鸡血管瘤型J亚群白血病的分子生物学诊断

针对福建省某鸡场的蛋鸡群发生产蛋下降和部分死亡,病鸡脚趾明显呈现血管瘤特征,运用实时荧光定量PCR方法对病鸡组织和血液样品进行检测,检出了J亚群白血病病毒(ALV-J);通过基因序列的比较分析,发现其氨基酸序列与国外及国内其他省份报导的ALV-J有很高的同源性,从病原分子生物学诊断角度证实福建省蛋鸡群中ALV-J的存在。     

木瓜愈伤组织中齐墩果酸的含量测定

利用木瓜芽孢进行愈伤组织的诱导,采用渗漉法对木瓜愈伤组织中的齐墩果酸进行了提取,然后用薄层层析法对齐墩果酸进行了分离,用比色法进行测定.筛选出最适合愈伤组织生长的培养基为,MS+2,4-D2.5 mg·L-1+6-BA 0.5 mg·L-1,木瓜愈伤组织中游离齐墩果酸的含量为0.078%.通过木瓜愈伤组织进行齐墩果酸的...     

区域水系与崩岗空间分布的关系

利用GIS空间叠加图层,通过野外实地调查取样,探讨了南方典型崩岗区(安溪县龙门镇)水系和崩岗的空间分布规律.结果表明,区域一级河流两侧斜坡几乎没有崩岗,二级河流两侧斜坡仅有少量崩岗发育,三级河流及其支流两侧和源头沟谷斜坡崩岗强烈发育.导致不同级别河流水系崩岗空间分布差异的原因是:一、二级河流长期处于侧向侵蚀状态中,其两...     

单一菌种与复合菌种益生菌制剂对蛋鸡生产效果研究

为了探讨单一菌种与复合菌种益生菌制剂对蛋鸡生产效果的影响。本研究以鸡源枯草芽孢杆菌、乳酸杆菌为益生菌株,利用发酵培养制备乳酸杆菌益生菌制剂、枯草芽孢杆菌益生菌制剂和乳酸杆菌—枯草芽孢杆菌复合益生菌制剂;在140日龄罗曼褐商品蛋鸡基础日粮上,按照质量比0.1%比例分别添加不同益生菌制剂,进行蛋鸡生产效果研究。结果表明,益生菌制剂可以显著提高平均蛋重、日产蛋量、产蛋率,降低料蛋比,提高蛋品质。与乳酸杆菌益生菌制剂、枯草芽孢杆菌益生菌制剂相比,乳酸杆菌—枯草芽孢杆菌复合益生菌制剂可以使平均蛋重分别显著提高1.87%、1.91%;平均日产蛋量分别提高了3.34%、2.77%;产蛋率分别提高1.56%、1.00%;料蛋比分别降低1.99%、1.49%; A级蛋比例分别提高9.16%、5.91% ;蛋黄比率分别提高7.71%、4.14%;每只鸡毛利润分别提高10.84%、7.73%;而在降低不合格蛋率、死淘率、平均日耗料量、蛋形指数和提高蛋比重、蛋壳厚度等方面,各益生菌制剂组差异不显著。因此,乳酸杆菌—枯草芽孢杆菌复合益生菌制剂对蛋鸡生产效果优于乳酸杆菌益生菌制剂、枯草芽孢杆菌益生菌制剂。     

宁南旱区苜蓿草地土壤水分和根系动态分布拟合曲线特征

试验以宁南旱区3、4、6、7、10、12年生紫花苜蓿草地为研究对象,利用空间替代时间的方法,分别对紫花苜蓿的生长年限与土壤水分、根系深度和土壤干层厚度之间关系作出拟合曲线方程,以揭示土壤水分、根系和土壤干层随苜蓿生长年限变化的动态规律.结果表明:不同生长年限苜蓿土壤剖面含水率分布与土壤深度之间均呈二次曲线函数关系,且随...     

一次强雷暴过程中的VWP产品特征分析

[目的]研究一次强雷暴过程中的VWP产品特征分析。[方法]在前人分析的基础上,对2008年4月8日湖南一次强雷暴发生前、维持发展中、消失前的不同阶段的VWP产品特征进行详细的分析。[结果]在雷暴出现前,低层＂ND＂层的破坏预示着水汽含量层的增厚,中层干冷空气的侵入导致了层结的不稳定;雷暴维持期间,低层强的垂直风切变会导致强雷暴发展,边界层冷空气渗透导致雷暴达到最强盛阶段,地面出现冰雹天气;雷暴消失前（17：59后）,整个风场转为连续＂ND＂层的一个趋势表明强对流云已远离雷达所在地,预示着雷暴结束。[结论]该研究为预报员做雷暴预警提供参考依据。     

森林土壤学研究

森林土壤由于分布范围广,成土因素各异,因而又是一个庞大的土壤系列,其中包括发育于不同气候条件和森林植被下的各具特点的不同土类。在中国,主要有灰化土、暗棕壤、棕壤、黄棕壤、红壤(黄壤)以及褐土等。本文主要进行森林土壤学研究。     

夏季高温蛋鸡养殖的成功实践

某年重庆地区遇上了夏季特大高温天气,地处巴南区境内的蛋鸡规模养殖场为此经历了一次严峻的考验。在此,笔者就此重庆地区夏季高温蛋鸡养殖的成功实践表诉于后,以供参考。     

蜂花粉在养鸡生产中的应用

蜂花粉是一种营养全面、具有多种生物活性物质的新型绿色饲料添加剂,具有增强畜禽机体免疫功能、营养促生长、肠道保健和提高动物源性食品质量与安全的功能。就蜂花粉分别在肉鸡、蛋鸡和种鸡生产过程中的应用研究进行了综述。     

Short-term response of the herbaceous layer within leave patches after harvest

Leave patches, uncut areas in a harvested forest, may conserve herbaceous layer species that decline after forest clearcutting. They may also serve to maintain source populations for the recolonization of the harvest area. The main objective of this study is to characterize the short-term response of the herbaceous layer to clearcut harvesting disturbance within and adjacent to leave patches. Four experimental 1 ha patches and two uncut references were established. Within each patch or reference, belts of five 1 m² quadrats were placed at 50 m and 5 m outside the patch, at 0 m (edge), and at 25 m, 35 m, and 50 m (centre) inside. These belts of quadrats were replicated at all four aspects (North, South, East, and West). Ground vegetation was sampled yearly for 1 year before harvest and 3 years after harvest in the experimental patches and for 2 consecutive years in the references. Some common residual species declined significantly in the clearcuts, but remained stable inside the patches in the short term (3 years after harvest). Overall species composition changed little in the patch interior and edge after harvest. Colonizing species (those that appeared in quadrats after harvest) in the patch exterior were predominantly early-seral species, whereas colonizers in the patch interior tended to be shade-tolerant forest species. Species were grouped a priori based on two habitat preferences (amounts of canopy cover and disturbed substrate). Species characteristic of a habitat with closed canopy and undisturbed substrates that decreased significantly over time in the patch exterior were negatively correlated with the amount of exposed mineral soil and open canopy. Species characteristic of a habitat with open canopy and disturbed substrates that increased significantly at the patch exterior showed a positive correlation with the amount of exposed mineral soil and open canopy. No species’ habitat preference groups declined significantly in the patch interior or edge over time. Based on species’ responses to forest operations, it appears that at least the common forest species are maintained in the short term within leave patches.